There are nine sports sponsored by the Texas Wesleyan University athletic department. The men's sports with traditional season in parenthesis are: Soccer (Fall), Basketball (Winter), Golf (Spring), and Baseball (Spring). The women's sports with traditional season in parenthesis are: Soccer (Fall), Volleyball (Fall), Basketball (Winter), and Softball (Spring).  Texas Wesleyan also features the most dominant Table Tennis team in the country.  Junior Varsity Baseball and Junior Varsity Basketball have also been added to increase the participation opportunities for students at Wesleyan.

The Director of Intercollegiate Athletics, is Mr. Kevin Millikan. The athletic department has a staff which includes an administrative assistant, sports information director, a faculty athletic representative, and a head athletic trainer. Each head coach is responsible for several different activities including teaching, coaching, recruiting, scheduling, budgeting, maintenance, fund raising, and team performance.

The athletic department is serviced by two student groups, the student trainers and cheerleaders. The student trainers are all in the academic major of sports medicine under the direction of Dr. Pam Rast. The head athletic trainer is Mr. Kyle Morgan. The student trainers service all nine sports by providing coverage for practices and game activities. The cheerleaders are a recognized student club on campus. Cheerleaders are selected based on tryouts and cheer primarily at men's and women's basketball games and provide organizational efforts for school wide pep rallies.

Texas Wesleyan University has been a member of the Red River Conference of the NAIA since the fall 2002. The member institutions are located in Texas, New Mexico, and Oklahoma and include SW Assemblies of God, Northwood, Texas College, Paul Quinn, Jarvis Christian, University of the Southwest, Bacone, Wiley, Huston-Tillotson, UT-Brownsville, and Langston.